Analysis of aseptic operation technology in biological laboratory
In the field of biology, the accuracy and reliability of experiments are very important for the influence of scientific research results. One of the keys to ensure the accuracy of experimental results is to ensure the aseptic environment during the experiment. This requires us to master and correctly implement aseptic operation technology.
First, what is aseptic operation?
Aseptic operation refers to an operation mode in which experimental equipment and samples are isolated from external pollution sources by various means (such as sterilization, disinfection, etc.) during biological experiments. Its main purpose is to avoid the influence of microorganisms or other impurities on the experimental results.
Second, the significance of aseptic operation
1. Ensure the accuracy of the experiment: aseptic operation can effectively eliminate the interference of external factors and make the experimental data more reliable and repeatable;
2. Avoid cross-contamination: When dealing with different kinds of samples, aseptic operation can effectively prevent cross-contamination, thus protecting the safety and health of experimenters;
3. Improve the efficiency of scientific research: once pollution occurs, it means that the whole experimental process must be restarted, which is time-consuming and laborious; On the other hand, if the whole process of sterility can be successfully realized, the work efficiency can be greatly improved.
Third, how to carry out the correct aseptic operation?
1. Preparation:
Clean hands and wear personal protective equipment (gloves, masks, etc.), and keep the experimental table clean and tidy;
Put all the instruments and equipment needed in clean bench, and carry out ultraviolet irradiation sterilization pretreatment on them;
All glassware should be sterilized by high-pressure steam before being put into clean bench for later use.
2. Operating steps:
(1) turn on the clean bench fan and ultraviolet lamp, and wait for a period of time to kill bacteria in the air;
(2) wiping the surface of the test tube cover with an alcohol cotton ball, then opening the cover and placing the test tube mouth obliquely downward above the flame for heating and sterilization;
(3) gently shaking the inoculation ring beside the flame to make it evenly heated until it turns red, and then taking a proper amount of the object to be inoculated with tweezers, transferring it to the culture medium and smearing it evenly;
(4) Close the test tube cover immediately after the corresponding steps, and turn off the flame to avoid unnecessary risks;
3. Safety precautions:
It is forbidden to touch any unsterilized object during the experiment;
Don't directly touch the articles that have been sterilized at high temperature to prevent scalding accidents;
Be sure to clean your hands and take off your personal protective equipment before leaving the experimental area.
Fourth, summary
In a word, strictly observing the standardized aseptic operation procedures in biological research work is not only a manifestation of self-responsibility, but also a necessary basic requirement for checking the quality of scientific research achievements that may involve human health in the future. I hope everyone can pay attention to it!
Rec. Prods.
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